Moscatilin Inhibits Lung Cancer Cell Motility and Invasion via Suppression of Endogenous Reactive Oxygen Species
Figure 6
Effect of moscatilin on migratory-related proteins. (a) H23 cells was treated with various nontoxic doses of moscatilin (0-1 μM) for 24 h and analyzed for protein expression by using western blot analysis as described under Section 2. Cells were collected and analyzed for phosphorylated FAK (Tyr 397), FAK proteins, phosphorylated Akt (Ser 473), Akt, phosphorylated-Erk1/2 (Thr202/Tyr204), Erk1/2, and Cdc42 proteins. Blots were reprobed with β-actin to confirm equal loading. (b) The immunoblot signals were quantified by densitometry and mean data from four independent experiments were presented. Values are means of samples ± SD. versus nontreated control cells. (c) A schematic diagram summarizes the inhibitory effect of moscatilin on lung cancer cell migration and invasion. Moscatilin suppresses ROS production and consequently attenuates the activation of FAK and Akt in H23 cells.
