Mass Spectrometry-Based Proteomics in Molecular Diagnostics: Discovery of Cancer Biomarkers Using Tissue Culture
Experimental outline of iTRAQ. Proteins are extracted from either tissue samples or cultured cells and subjected to proteolytic digestion. The digested peptides are then labelled with isobaric tags followed by the pooling of the samples. The samples are then fractioned through SCX followed by tandem mass spectrometry analysis.