BioMed Research International / 2013 / Article / Fig 1

Research Article

α-Ketoglutarate Accumulation Is Not Dependent on Isocitrate Dehydrogenase Activity during Tellurite Detoxification in Escherichia coli

Figure 1

In situ ICDH and GDH activity and transcriptional level of gdhA and icdA genes in tellurite-exposed E. coli. ICDH (a) and (b) GDH activity was assayed after fractionating extracts from tellurite-treated cells by native gradient polyacrylamide gels as described in methods. Representative gels are shown. (c) Transcriptional level of gdhA and icdA genes in tellurite-exposed E. coli. Numbers represent the mean of 3 independent trials. The relative expression was normalized to that of the rpoD gene.
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