α-Ketoglutarate Accumulation Is Not Dependent on Isocitrate Dehydrogenase Activity during Tellurite Detoxification in Escherichia coli
In situ ICDH and GDH activity and transcriptional level of gdhA and icdA genes in tellurite-exposed E. coli. ICDH (a) and (b) GDH activity was assayed after fractionating extracts from tellurite-treated cells by native gradient polyacrylamide gels as described in methods. Representative gels are shown. (c) Transcriptional level of gdhA and icdA genes in tellurite-exposed E. coli. Numbers represent the mean of 3 independent trials. The relative expression was normalized to that of the rpoD gene.
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