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BioMed Research International
Volume 2013, Article ID 790842, 11 pages
Research Article

Late Adherent Human Bone Marrow Stromal Cells Form Bone and Restore the Hematopoietic Microenvironment In Vivo

1Clinical and Basic Research Division, National Institute of Traumatology and Orthopaedics, Avenida Brasil 500, 20940-070 Rio de Janeiro, RJ, Brazil
2Institute of Biomedical Sciences, Federal University of Rio de Janeiro, 21941-970 Rio de Janeiro, RJ, Brazil
3Laboratory of Nuclear Instrumentation, COPPE, Federal University of Rio de Janeiro, 21941-970 Rio de Janeiro, RJ, Brazil
4VA Greater Los Angeles Health Care System, Los Angeles, CA 90073, USA
5University of California, Los Angeles, CA 90095, USA

Received 30 November 2012; Revised 23 February 2013; Accepted 14 March 2013

Academic Editor: George E. Plopper

Copyright © 2013 Verônica Fernandes Vianna et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Bone marrow stromal cells (BMSCs) are a valuable resource for skeletal regenerative medicine because of their osteogenic potential. In spite of the very general term “stem cell,” this population of cells is far from homogeneous, and different BMSCs clones have greatly different phenotypic properties and, therefore, potentially different therapeutic potential. Adherence to a culture flask surface is a primary defining characteristic of BMSCs. We hypothesized that based on the adherence time we could obtain an enriched population of cells with a greater therapeutic potential. We characterized two populations of bone marrow-derived cells, those that adhered by three days (R-cells) and those that did not adhere by three days but did by six days (L-cells). Clones derived from L-cells could be induced into adipogenic, chondrogenic, and osteogenic differentiation in vitro. L-cells appeared to have greater proliferative capacity, as manifested by larger colony diameter and clones with higher CD146 expression. Only clones from L-cells developed bone marrow stroma in vivo. We conclude that the use of late adherence of BMSCs is one parameter that can be used to enrich for cells that will constitute a superior final product for cell therapy in orthopedics.