BioMed Research International / 2013 / Article / Fig 1

Research Article

Helminth Protein Vaccine Induced Follicular T Helper Cell for Enhancement of Humoral Immunity against Schistosoma japonicum

Figure 1

The enhancement of humoral immune response in mice immunized with SjGST-32/FK506. The BALB/c mice were immunized twice with SjGST-32/FK506 in a 2-week interval. (a) Seven days after the second injection, the serum was collected for IgG and IgG1 tests by ELISA. Bar, mean, and SD from 3 independent experiments each using three mice per group. indicates statistically very significant between the pairs. (b) Seven days after the second injection, B cells were isolated from splenocytes and the purity of B cells was above 93%. B cells were labeled with CFSE and performed for proliferation assay. The isolated B cells were cultured with SjGST-32 or LPS as stimulator for 72 hours and were analyzed by flow cytometry. Shown in each panel is 1 of at least 3 experiments with similar results. (c) The summary of B-cell proliferation; bar, mean, and SD from 3 independent experiments each using at least three mice per group ( ). For statistical analysis, mice immunized with SjGST-32/FK506 were compared with those in other groups and ANOVA was used; . **Indicates that the difference is very significant at the 0.01 level

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