BioMed Research International / 2013 / Article / Fig 3

Research Article

Helminth Protein Vaccine Induced Follicular T Helper Cell for Enhancement of Humoral Immunity against Schistosoma japonicum

Figure 3

The IL-21R expression of GC B cells in mice immunized with SjGST-32/FK506. The BALB/c mice were immunized twice with SjGST-32/FK506 in a 2-week interval. (a) Seven days after the second injection, the splenocytes were prepared and stained with anti-mouse B220, GL-7, and CD95 mAbs. The samples were analyzed by flow cytometry. The B220+ cells were gated for analysis of GCs B cells and the data are shown as the percentage of GC B cells (B220+CD95+GL-7+) in total B cells (B220+). Shown in each panel is 1 of at least 3 experiments with similar results. The graphic result of the percentage of GC B cells was shown. (b) Seven days after the second injection, the splenocytes were prepared and stained with anti-mouse B220, IL-21R Abs, GL-7C, and CD95 mAbs. The B220+IL-21R+ cells were gated and the data are shown as the percentage of IL-21R expression (B220+IL-21R+CD95+GL-7+) in total GC B cells (B220+CD95+GL-7+). Shown in each panel is 1 of 3 experiments with similar results. The graphic result of the percentage of IL-21R+GC B cells was shown; bar, mean, and SD from 3 independent experiments each using three mice per group. For statistical analysis, mice immunized with SjGST-32/FK506 were compared with those in other groups and ANOVA was used; .
798164.fig.003a
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798164.fig.003b
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