BioMed Research International / 2013 / Article / Fig 4

Research Article

Helminth Protein Vaccine Induced Follicular T Helper Cell for Enhancement of Humoral Immunity against Schistosoma japonicum

Figure 4

The expression of IL-21R on memory B cells was increased in mice immunized with SjGST-32/FK506. The BALB/c mice were immunized twice with SjGST-32/FK506 in a 2-week interval. Forty-nine days after the second injection, the splenocytes were prepared. (a) The samples were stained with anti-mouse B220-APC and CD27-PE Abs for memory B cells. The B220+ cells were gated and the data are shown as the percentage of memory B cells (B220+CD27+) in total B cells (B220+). Shown in each panel is 1 of at least 3 experiments with similar results. (b) The splenocytes were stained with anti-mouse B220, IL-21R, and CD27 Abs for IL-21R expression on memory B cells. The samples were analyzed by flow cytometry. The B220+ cells were gated and the data are shown as the IL-21R expression (B220+CD27+IL-21R+) on memory B cells (B220+CD27+). Shown in each panel is 1 of 3 experiments with similar results; bar, mean, and SD from 3 independent experiments each using at least three mice per group ( ). For statistical analysis, mice immunized with SjGST-32/FK506 were compared with those in other groups and ANOVA was used; .
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798164.fig.004b
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