Research Article

Knockout of Density-Enhanced Phosphatase-1 Impairs Cerebrovascular Reserve Capacity in an Arteriogenesis Model in Mice

Figure 3

(a) Endothelial cells were transfected with DEP-1 siRNA or nontargeting siRNA for 48 hours. Representative microphotographs were taken 48 hours after transfection. Protein lysates were WGA precipitated and immunoblotted against DEP-1. Beta-actin immunoblots served as loading control. Representative qPCR analyses of DEP-1 (b) and PDGF-B (c) expressions in endothelial cells 48 hours after DEP-1 siRNA knockdown are depicted ( ).
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(c)