Figure 3: (a) Endothelial cells were transfected with DEP-1 siRNA or nontargeting siRNA for 48 hours. Representative microphotographs were taken 48 hours after transfection. Protein lysates were WGA precipitated and immunoblotted against DEP-1. Beta-actin immunoblots served as loading control. Representative qPCR analyses of DEP-1 (b) and PDGF-B (c) expressions in endothelial cells 48 hours after DEP-1 siRNA knockdown are depicted ( ).