Figure 4: (a) Collateral perfusion index after FAO. Wild-type and DEP-1−/− animals were perfused with fluorescent microspheres at different compression intensities after maximal vasodilatation. Limb muscles were lysed, and microsphere counts (FACS) were normalized to a defined number of blue spheres as outlined in Materials and Methods (wild-type , knockout ). (b) Representative micro-CT-based images of the femoral artery and interconnecting peripheral hindlimb vessels (red arrows, left) as well as 3D images for visualization of vascular intratissue characteristics (right). (c) Quantifications of external peripheral vessel diameter measurements are shown.