BioMed Research International / 2013 / Article / Fig 2

Research Article

A Lys49 Phospholipase , Isolated from Bothrops asper Snake Venom, Induces Lipid Droplet Formation in Macrophages Which Depends on Distinct Signaling Pathways and the C-Terminal Region

Figure 2

MT-II induces formation of LDs in peritoneal macrophages in culture.(a) Effect of selected concentrations of MT-II on formation of LDs in macrophages which were incubated with various concentrations of MT-II or with RPMI (Control) for 1 h. (b) Time-course of MT-II-induced LD formation. Macrophages were incubated with MT-II (0.8 μM) or RPMI (Control) for 1, 3, 6, 12, or 24 h. LDs were quantified using light microscopy after osmium staining. LDs after osmium staining observed in control (c) or in cells stimulated with MT-II (0.8 μM) for 1 h (d), 12 h (e), or 24 h (f). Each bar represents the mean ± S.E.M. of the number of LDs/cell in 50 counted cells. Values represent means ± S.E.M. for three to five animals. * compared with control cells; # compared with cells stimulated by 0.2 or 0.4 μM of MT-II; + compared with cells stimulated by MT-II (0.8 μM) for 1, 3, 6 and 12 h.

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