BioMed Research International / 2013 / Article / Fig 4

Research Article

A Lys49 Phospholipase , Isolated from Bothrops asper Snake Venom, Induces Lipid Droplet Formation in Macrophages Which Depends on Distinct Signaling Pathways and the C-Terminal Region

Figure 4

Effects of peptides corresponding to selected regions of MT-II molecule on LD formation. (a) Peritoneal macrophages were incubated with p115-129 (250 μg/mL) or pScr (250 μg/mL) or p60–71 (250 μg/mL) or RPMI (control) for 3 h. LDs were quantified using light microscopy after osmium staining; (b) cells were incubated with p115–129 (250 μg/mL) or pScr (250 μg/mL) or p60–71 (250 μg/mL) or PEM2 (150 μg/mL) or p115–W3 (150 μg/mL) or RPMI (control) for 3 h, after which cytotoxicity was assessed by the tetrazolium-based (MTT) colorimetric assay; (c) effect of MT-II (0.8 μM) on formation of LDs in macrophages in a Ca2+ containing medium or Ca2+-free, EGTA (200 μM)-Sr2+-containing medium. LDs were quantified using light microscopy after osmium staining. Each bar represents the mean ± S.E.M. of the number of LDs/cell in 50 counted cells. Values represent means ± S.E.M. for three to five animals. * compared with control cells.

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