Research Article

Analytical Variables Affecting Analysis of F2-Isoprostanes and F4-Neuroprostanes in Human Cerebrospinal Fluid by Gas Chromatography/Mass Spectrometry

Figure 6

Comparison on the chromatograms for the 5 consecutive injections of the same pooled M-CSF sample processed for F4-NPs analysis (CSF-1 to CSF-5) at m/z 593.5 without (left panel) and with (right panel) additional 30-min holding of the column at 280°C at the end of data acquisition. For better viewing of the peaks of F4-NPs and the adjacent peaks, the chromatograms in the range of 4.8 min to 7.8 min were compared. The peaks for F4-NPs quantification are indicated by the braces.
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