Analytical Variables Affecting Analysis of F₂-Isoprostanes and F₄-Neuroprostanes in Human Cerebrospinal Fluid by Gas Chromatography/Mass Spectrometry

Table 3

Results of quantification of F₂-IsoPs from 5 consecutive injections of the same pooled sample.

	F2-IsoPs levels (pg/mL)
	No additional holding	30-min holding
CSF-1	56.1	54.8
CSF-2	55.5	55.3
CSF-3	54.8	55.2
CSF-4	54.1	56.4
CSF-5	53.8	55.5
Mean ± SD	54.9±1.0	55.4±0.6
(CV%)	(1.8%)	(1.1%)

The sample number was designated according to the sequence of injection. There was no statistical significance between two sets of data obtained by two different methods (𝑝=0.281 under assumption of equal variance).