HPLC Quantitative Analysis of Rhein and Antidermatophytic Activity of Cassia fistula Pod Pulp Extracts of Various Storage Conditions

Table 1

The content of rhein in the decoction extract and antifungal activity against dermatophytes of the decoction extract and its hydrolyzed mixture containing anthraquinone aglycones of C. fistula pod pulp kept under various storage conditions of stability studies.

Time (month)	Packaging	Storage condition	Rhein content^a; (% w/w)	T.  mentagrophytes   DMST 19735  and T. mentagrophytes; MIC (µg/mL)		T. rubrum; MIC (µg/mL)		M. gypseum; MIC (µg/mL)
				Decoction extract	Hydrolyzed mixture	Decoction extract	Hydrolyzed mixture	Decoction extract	Hydrolyzed mixture
0			0.0823±0.001; (100.00%)^b	>1000	500	>1000	250	>1000	500
3	Glass vial	Real time	0.0829±0.001; (100.66%)	>1000	500	>1000	250	>1000	500
	Aluminum foil	Real time	0.0815±0.001; (98.96%)	>1000	500	>1000	250	>1000	500
	Glass vial	Accelerated	0.0817±0.003; (99.25%)	>1000	500	>1000	250	>1000	500
	Aluminum foil	Accelerated	0.0809±0.002; (98.23%)	>1000	500	>1000	250	>1000	500
6	Glass vial	Real time	0.0791±0.002; (96.11%)	>1000	500	>1000	250	>1000	500
	Aluminum foil	Real time	0.0794±0.001; (96.46%)	>1000	500	>1000	250	>1000	500
	Glass vial	Accelerated	0.0790±0.001; (95.95%)	>1000	500	>1000	250	>1000	500
	Aluminum foil	Accelerated	0.0788±0.005; (95.69%)	>1000	500	>1000	250	>1000	500

aExpressed as mean ± SD (𝑛=3) and bcontent of rhein in C. fistula decoction extract calculated as 100% at day 0. Real time = 30°C ± 2°C/75% RH ± 5% RH. Accelerated = 40°C ± 2°C/75% RH ± 5% RH.