The Cytotoxic Effect of Magainin II on the MDA-MB-231 and M14K Tumour Cell Lines

Table 2

The percentages of living, apoptotic, and dead mammary adenocarcinoma cells were determined by flow cytometry, as shown in this table, where Q1 quadrant includes dead cells (7-AAD positive), Q2 quadrant contain late apoptotic (7-AAD positive, Annexin V positive), and Q4 quadrant contains early apoptotic cells (Annexin V positive, 7-AAD negative). Viable cells (7-AAD negative, Annexin V negative) were enclosed in the Q3 quadrant.

MDA-MB231; 5000 events/well	Q1—dead cells (7-AAD+)	Q2—late apoptosis cells (7-AAD+ Annexin V+)	Q3—living cells; (7-AAD− Annexin V−)	Q4—early apoptosis cells; ( Annexin V+ 7-AAD−)
	%	%	%	%
Negative control (NC)	1.8	3	90.5	4.7
Magainin II 120 μM (M120)	1.4	47.5	36.3	14.8
Magainin II 60 μM (M60)	0.8	19.6	76.2	3.4