Development of a Novel System for Mass Spectrometric Analysis of Cancer-Associated Fucosylation in Plasma α1-Acid Glycoprotein
Table 1
Relative abundances of N-glycans with bi-, tri-, and tetra-antennary chains and their fucosylated ones in plasma AGP from healthy controls and colorectal cancer patients.
Relative abundance (%)#
Glycan structure
Numbers of
Normal
Cancer
P value
[GlcNAc]
[Fuc]
()
()
Bi-antennary
2
0
36.12 ± 15.52
42.07 ± 14.03
n.s.
3
0–2
47.10 ± 12.69
42.54 ± 13.03
n.s.
Tri-antennary
3
0
37.44 ± 12.57
25.84 ± 9.76
<0.001
3
1
8.26 ± 3.63
14.60 ± 5.42
<0.001
3
2
1.40 ± 1.05
2.10 ± 1.06
<0.001
Fuc-tri-antennary
3
1-2
9.66 ± 3.02
16.69 ± 5.42
<0.001
4
0–3
15.03 ± 7.69
13.15 ± 4.08
n.s.
4
0
7.95 ± 4.15
5.41 ± 2.19
<0.001
Tetra-antennary
4
1
1.42 ± 1.16
2.32 ± 1.30
<0.001
4
2
0.57 ± 0.50
0.66 ± 0.57
n.s.
4
3
0.29 ± 0.28
0.34 ± 0.36
n.s.
Fuc-tetra-antennary
4
1–3
2.28 ± 1.65
3.32 ± 1.82
<0.01
5–7
0–6
1.63 ± 1.24
1.58 ± 0.91
n.s.
Tri- + tetra-antennary
3–7
0
62.12 ± 17.09
55.69 ± 14.88
n.s.
Fuc-(tri- + tetra-antennary)*
3–7
1–6
18.68 ± 6.33
23.02 ± 5.7
<0.001
based on the hit area to the total one. *FUCAGP: fucosylated (tri + tetra)-antennary glycans/total glycans × 100.
