High Expression and Purification of Amino-Terminal Fragment of Human Amyloid Precursor Protein in Pichia pastoris and Partial Analysis of Its Properties

Table 1

Primer sequence used for PCR amplification of N-APP.

5′ Forward primer; 2 𝜇g (312 pmoles),	5′CCG CTCGAG AAAAGA↓CTGGAGGTACCCACTGATGGTAATG3′
lyophilized	Xho I   Kex2 signal cleavage
3′ Reverse primer	5′GGAATTCCTTATTAATGATGATGATGATGATGGGATCCTCTTGGAACCAACTCTTCCACAGACTCTGT; GGTGGTG3′
2 𝜇g (314 pmoles),	EcoR I Double stop codon 6His-tag  Ser Gly ↓ Arg Pro Val Leu
lyophilized	(Thrombin cleavage site)