High Expression and Purification of Amino-Terminal Fragment of Human Amyloid Precursor Protein in Pichia pastoris and Partial Analysis of Its Properties

Table 2

Summary of N-APP purification.

Purification step	Total volume (mL)	Total protein (mg)	Purity (%)^a	Recovery (%)
Culture supernatant	400	204	35	100
Q-Sepharose FF	50	43.1	80	25.9
Ultrafiltration	8	30.4	87	70.5
Sephacryl S-200	13.3	23.6	96	77.6

aAccording to densitometric analysis of SDS-PAGE.