Differential Expression of Na+/H+-Exchanger (NHE-1, 2, and 4) Proteins and mRNA in Rodent’s Uterus under Sex Steroid Effect and at Different Phases of the Oestrous Cycle
Figure 6
Immunolocalization of NHE4 in steroid replaced ovariectomized rats and in rats at different phases of the oestrous cycle. Bilateral distribution of NHE4 was observed following E treatment and at Ps and Es stages of the cycle, however the expression was reduced under P dominance. Magnifications of 10X and 100X (in the upper right corner). 0.2E: 0.2 μg 17β-oestradiol, 2E: 2 μg 17β-oestradiol, 20E: 20 μg 17β-oestradiol, 50E: 50 μg 17β-oestradiol, P: progesterone, E + P: 0.2 μg 17β-oestradiol + progesterone. Ps: proestrus, Es: estrus, Ms: metestrus, Ds: diestrus. rats per group. Incubation with a non-immune peptide was used as a negative control to check for the specificity of the antibodies. No staining was observed in this experiment.
