BioMed Research International / 2013 / Article / Fig 3

Research Article

A New Dual-Promoter System for Cardiomyocyte-Specific Conditional Induction of Apoptosis

Figure 3

Apoptosis induction and cell survival. 48 hours after transduction with pNRTA vector, apoptosis was induced in HL-1 cells following the exposure to Tet as indicated. Cells were then washed and trypsinized, and live cells were counted by trypan blue exclusion. (a) Number of viable, NRTA-transduced, HL-1 cells after exposure to the indicated amount of Tet for 24 hours. (b) Number of viable, untransduced, HL-1 cells after exposure to the indicated amount of Tet for 24 hours. (c) Number of viable HL-1 cells (transduced and untransduced) after exposition at the indicated amount of Tet for 24 hours. (d) HL-1 cells transduced with pNRTA vector. (e) HeLa cells transduced with pNRTA vector. (f) HL-1 cells transduced with empty pTY2 vector. (g) HeLa cells, transduced with empty pTY2 vector. Cell survival in panels (d) to (g) are expressed as a fraction of live cells over total cells. : cells unexposed with tetracycline; +Tet: cells exposed to 1 μg/mL tetracycline.
845816.fig.003a
(a)
845816.fig.003b
(b)
845816.fig.003c
(c)
845816.fig.003d
(d)
845816.fig.003e
(e)
845816.fig.003f
(f)
845816.fig.003g
(g)