Figure 1: Bone marrow-derived precursor cells from childhood acute leukemia express Toll-like receptors (TLRs). Bone marrow CD34+ cells were purified by magnetic separation and analyzed for TLRs transcription by RT-PCR (a). Positive controls resulted from TLR-expressing plasmids, while the negative control was DEPC H2O. Transcript expression levels in various acute leukemia subtypes relative to their normal counterpart were tabulated after density analyses (b). 18 s rRNA subunit was used as housekeeping gene. NBM: normal bone marrow; B-ALL: B-cell precursor acute lymphoblastic leukemia; C-ALL: congenital acute lymphoblastic leukemia; T-ALL: T-cell acute lymphoblastic leukemia; M-ALL: mixed acute lymphoblastic leukemia; AML: acute myeloid leukemia.