Rotenone Upregulates Alpha-Synuclein and Myocyte Enhancer Factor 2D Independently from Lysosomal Degradation Inhibition

Figure 7

Effect of ammonium chloride (NH₄Cl) on alpha-synuclein (asyn), MEF2D, lamp2A, and hsc70 mRNA levels. Relative quantification (RQ) of asyn, MEF2D, lamp2A, and hsc70 mRNA calculated as ratio to beta-actin in SH-SY5Y treated with 5 and 10 mM NH₄Cl for 24 hours, expressed as fold change versus vehicle (RQ = 1). 𝑁=4, repeated measures ANOVA test, followed by Dunnett’s post-test; ^∗𝑃<0.05; ^∗∗𝑃<0.01 versus vehicle.