Table 1: Optimum conditions used for enzymatic proteolysis of stone fish.

Enzyme Buffer (50 mM) pH Temperature (°C) Alcalase Borate buffer 7.9 55 Bromelain Acetate buffer 4.5 50 Flavourzyme Phosphate buffer 6.5 50 Papain Phosphate buffer 6.5 55 Pepsin Acetate buffer 3.6 37 Trypsin Phosphate buffer 6.9 37