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BioMed Research International
/
2013
/
Article
/
Tab 1
Research Article
The Improvement of The Endogenous Antioxidant Property of Stone Fish (
Actinopyga lecanora
) Tissue Using Enzymatic Proteolysis
Table 1
Optimum conditions used for enzymatic proteolysis of stone fish.
Enzyme
Buffer (50 mM)
pH
Temperature (°C)
Alcalase
Borate buffer
7.9
55
Bromelain
Acetate buffer
4.5
50
Flavourzyme
Phosphate buffer
6.5
50
Papain
Phosphate buffer
6.5
55
Pepsin
Acetate buffer
3.6
37
Trypsin
Phosphate buffer
6.9
37