STAT6 siRNA Matrix-Loaded Gelatin Nanocarriers: Formulation, Characterization, and Ex Vivo Proof of Concept Using Adenocarcinoma Cells

Figure 2

Preparation of S6S-GNC. The 1% w/v aqueous gelatin solution is incubated with the STAT6 siRNA for 10 min at 35°C, and then ethanol and crosslinker are added dropwise at a stirring rate of 600 rpm at 35°C for 1 hr, at which point the stirring rate is reduced to 200 rpm. After approximately 4 h, the ethanol is completely evaporated, and STAT6 siRNA loaded gelatin nanocarriers remain in a colloidal suspension in water or PBS pH 7.4. The resultant nanoparticles were collected by centrifugation and resuspended for subsequent characterization or lyophilization in the presence of 1% w/w lactose monohydrate.