Research Article

STAT6 siRNA Matrix-Loaded Gelatin Nanocarriers: Formulation, Characterization, and Ex Vivo Proof of Concept Using Adenocarcinoma Cells

Figure 8

Agarose gel electrophoretic mobility shift assay. The scrambled siRNA control, scrambled siRNA treated with RNAse control, S6S-GNC, S6S-GNC treated with RNAse, filtrate, filtrate treated with RNAse, and the placebo-GNC treated with RNAse were loaded onto a 1% w/v agarose gel and electrophoresed at a constant voltage of 70 V until the bromophenol blue marker bands were well separated. This study was performed to examine the stability of the encapsulated siRNA due to preparation conditions, as well as the stability in the presence of RNAse. The arrow head indicates the distance traveled by the cleaved siRNA fragments.
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