Expression of Pluripotency and Oocyte-Related Genes in Single Putative Stem Cells from Human Adult Ovarian Surface Epithelium Cultured In Vitro in the Presence of Follicular Fluid

Table 1

Clinical data of all five patients with POF included into this study, which were documented at the Department of Obstetrics and Gynecology, University Medical Centre Ljubljana. All patients had increased serum levels of gonadotropins FSH (normal: <11.3 IU/L) and LH (normal: <11.6 IU/L) and thin endometrium.

Clinical data	Patients
	P1; (R.A.)	P2; (G.K.)	P3; (M.S.)	P4; (G.A.)	P5; (P.R.A.)
Age (years)	39	40	21	31	39
FSH (IU/L)	89.1	65.8	11.8	162.0	67.8
LH (IU/L)	23.3	23.4	10.1	59.0	37.8
Prolactin (mg/L)	12	4.6	16.4	9.3	/
Estradiol (nmol/L)	<0.073	0.08	0.2	/	/
Inhibin B (ng/L)	<10	<10	52.4	>10	/
S-AMH (mg/L)	0.00	0.00	0.89	0.00	/
Karyotype	Abnormal; (mosaic 45X, 47XXX, 48XXXX, 46XX)	Normal; (no FMR1-fragile X mutation)	Normal; (no FMR1-fragile X mutation)	Normal; (no FMR1-fragile X mutation)	Normal; (no FMR1-fragile X mutation)
Ovarian cortex histology	Inclusion cysts, corpora albicantia, focal ovarian surface epithelium, no follicles or oocytes	Inclusion cysts, corpora albicantia, simple or stratified ovarian surface epithelium, no follicles or oocytes	Simple columnar ovarian surface epithelium, several primordial follicles	Simple cuboidal ovarian surface epithelium, corpus luteum in regression, no follicles or oocytes	Simple cuboidal ovarian surface epithelium,; no follicles or oocytes
Antiovarian antibodies	No	No	Yes	Yes	No
Premature ovarian failure (POF)	Secondary (previous birth of a child), irregularities of menstrual cycles, thin endometrium	Primary; (no children), irregular menstrual cycles, thin endometrium	Primary; (no children), irregular menstrual cycles, thin endometrium	Primary; (no children), amenorrhea, small left ovary, thin endometrium	Secondary (pregnancy ended in spontaneous abortion),; amenorrhea, thin endometrium