Screening of a Protein That Interacts with the Matrix Attachment Region-Binding Protein from Dunaliella salina
Figure 4
GST pull-down assay demonstrating the in vitro binding between DMBP-1 and DMBP-2. Purified His-DMBP-2 protein was added to GST. GST-DMBP-1 was immobilized on glutathione sepharose beads. His-DMBP-2 fusion protein at 10% was loaded as input. The interacting complexes were subsequently eluted and separated by SDS-PAGE (12%). (a) The immunoblot with monoclonal anti-GST antibody showed the presence of the GST-DMBP-1 fusion protein co-eluting with His-DMBP-2 from the glutathione sepharose beads. (b) The immunoblot with monoclonal anti-His antibody showed the presence of His-DMBP-2 coeluting with GST-DMBP-1 from glutathione sepharose beads. The presence of His-DMBP-2 in the input was also confirmed.
