p55PIK Transcriptionally Activated by MZF1 Promotes Colorectal Cancer Cell Proliferation

Figure 1

Main p55PIK promoter activity conferred by −1243/−840 upstream region sequence. (a) Series of 5′ end p55PIK promoter recombinant reporter plasmids; (b) DNA fragments with different lengths but with the same 3′ end of p55PIKpromoter cloned into pGL3-Basic reporter plasmids; (c, d) Relative luciferase activity (RLA) of p55PIK reporter plasmids was examined with Dual-Luciferase reporter assay in HepG2 and HeLa cell lines. Data are shown as mean ± SD (𝑛=4; ^∗∗𝑃<.01, per Student’s 𝑡 -test.)

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