Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells

Figure 3

Affinity chromatography of MG protein extracts. MG proteins were purified from extracts of different Ae. aegypti strains (DMEB, DS3, IBO-11, or Mori) by affinity chromatography using DENV-2, -1, -4 or rE2-DIII-Sepharose 4B column as described in Section 2. Midgut proteins were eluted from DENV-2-Sepharose 4B columns with buffer E containing 1 M NaCl (lines 1-2), or 0.1 M Glycine pH 2.7 (lines 3-4) and from rE2-DIII-Sepharose 4B column with 1 M NaCl (line 5) or 0.1 M Glycine pH 2.7 containing 0.5 M NaCl (line 6-7). Aliquots of 500 μL were collected from each column and proteins were acetone-precipitated and separated by 10% SDS-PAGE and Coomassie Brilliant Blue or silver stained. The apparent molecular weights of these proteins are shown on the right side. Molecular weight markers are shown on the left side.