Proteomic Identification of Dengue Virus Binding Proteins in Aedes aegypti Mosquitoes and Aedes albopictus Cells

Table 1

Mosquito proteins bound to DENV.

DENV-Sepharose 4B*	Cell/tissue expression/gel slice**	Name****	Accession number	Accession swissprot	Predicted mass (Da)	Size; (aa)	Mascot score	Theoretical isoelectric point*	Number of peptides	Protein coverage (%)
DENV-2	C6/36; 57 kDa	Enolase^1	gi∣157121051∣ref∣XP_001653750∣	Q17KK5	46621	433	76.1	5.6	4	15.9
DENV-2	C6/36; 67 kDa	Enolase^2	gi∣157121051∣ref∣XP_001653750∣	Q17KK5	46621	433	60.5	5.6	1	7.4
DENV-2	DS3 (extract)	Enolase^3	gi∣157121051∣ref∣XP_001653750∣	Q17KK5	46621	433	20.2	5.6	1	1.4
None	DMEB***; 67 kDa	Enolase^4	gi∣157121051∣ref∣XP_001653750∣	Q17KK5	46621	433	75	5.6	2	13
DENV-1	DMEB and DS3; 67 kDa	Beta-adrenergic receptor kinase^1	gi∣157114479∣ref∣XP_001652291∣	Q174J9	66217	580	40/26.9	6.74	1	2.6
DENV-4	C6/36; 67 kDa	Beta-adrenergic receptor kinase^2	gi∣157114479∣ref∣XP_001652291∣	Q174J9	66217	580	40/26.9	6.74	1	2.6
DENV-2	C6/36; 57 kDa	Translation elongation factor EF-1 alpha/Tu^1	gi∣94468780∣gb∣ABF18239.1∣	Q1HR88	50,473	463	82.9	9.61	1	5.4
DENV-2	MORI; (extract)	Translation elongation factor EF-1 alpha/Tu^2	gi∣94468780∣gb∣ABF18239.1∣	Q1HR88	50,473	463	21.8	9.61	1	1.7
None	IBO-11; 57 kDa	Translation elongation factor EF-1 alpha/Tu^3	gi∣94468780∣gb∣ABF18239.1∣	Q1HR88	50,473	463	63	9.61	2	10.8
DENV-4	C6/36; 80 kDa	Cadherin	gi∣157115805∣ref∣XP_001658290∣	Q17LY6	186427	1653	52.0	4.74	1	1%

∗Affinity chromatography was performed with DENV-2, -1, or -4.
**Bands of interest were excised at the molecular weight of interest (57 or 67 kDa).
***Total extract of MGs from Ae. aegypti mosquitoes strain DMEB was separated by SDS-PAGE and the band with apparent molecular weight of 67 kDa was excised for a further analysis.
****Superscript number in the name of the protein indicates the number of the experiment.