Figure 3: Alveolar bone resorption through TRAP(+) osteoclast identification. (a) Mesiodistal histological sections illustrate TRAP(+) cells on distal surfaces. The relative height of the alveolar bone crest (ABC) in relation to the cementoenamel junction (CEJ) is shown to decrease with duration of ligation. (b) Magnified images of 3A show alveolar bone crest (ABC), coronal, and apical regions of distal surfaces across time points and between control and ligated complexes (i–ix). The number of multinucleated osteoclasts (OC) located along the bone perimeter was counted in coronal and apical root segments on mesial (x) and distal (xi) sides. *Statistically significant difference at 95% confidence interval was observed. Alveolar bone (AB), periodontal ligament (PDL), dentin (D), and secondary cementum (SC).