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BioMed Research International
Volume 2013 (2013), Article ID 878374, 19 pages
Research Article

Reproducibility of NMR Analysis of Urine Samples: Impact of Sample Preparation, Storage Conditions, and Animal Health Status

1numares GROUP, Josef Engert Straße 9, 93053 Regensburg, Germany
2Institute of Biophysics and Physical Biochemistry and Centre of Magnetic Resonance in Chemistry and Biomedicine, University of Regensburg, 93040 Regensburg, Germany
3Department of Computer Science, Technical University Dortmund, Otto-Hahn-Straße 16, 44221 Dortmund, Germany
4Group General Pharmacology, Department Drug Discovery Support, Boehringer-Ingelheim Pharma GmbH & Co. KG, 88397 Biberach an der Riss, Germany

Received 25 February 2013; Revised 21 May 2013; Accepted 22 May 2013

Academic Editor: Anita M. Oberbauer

Copyright © 2013 Christina Schreier et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Introduction. Spectroscopic analysis of urine samples from laboratory animals can be used to predict the efficacy and side effects of drugs. This employs methods combining 1H NMR spectroscopy with quantification of biomarkers or with multivariate data analysis. The most critical steps in data evaluation are analytical reproducibility of NMR data (collection, storage, and processing) and the health status of the animals, which may influence urine pH and osmolarity. Methods. We treated rats with a solvent, a diuretic, or a nephrotoxicant and collected urine samples. Samples were titrated to pH 3 to 9, or salt concentrations increased up to 20-fold. The effects of storage conditions and freeze-thaw cycles were monitored. Selected metabolites and multivariate data analysis were evaluated after 1H NMR spectroscopy. Results. We showed that variation of pH from 3 to 9 and increases in osmolarity up to 6-fold had no effect on the quantification of the metabolites or on multivariate data analysis. Storage led to changes after 14 days at 4°C or after 12 months at −20°C, independent of sample composition. Multiple freeze-thaw cycles did not affect data analysis. Conclusion. Reproducibility of NMR measurements is not dependent on sample composition under physiological or pathological conditions.