Identification and Characterization of Cyclic AMP Response Element-Binding Protein H Response Element in the Human Apolipoprotein A5 Gene Promoter

Figure 4

CREBH binds to APOA5 promoter. HepG2 cells were subjected to formaldehyde cross-linking, and chromatin fragments were prepared by sonication and immunoprecipitated anti-CREBH antibody. Promoter sequences containing the CREBHRE sequence were analyzed by PCR using primer sets specific to the human APOA5 promoter. As a negative control, primers were used to amplify the GAPDH promoter region which was not expected to interact with the CREBH. Cell lysate solution (5%) in ChIP dilution buffer was kept aside as “input.” Three separate experiments were carried out, and a representative example of the result is shown.