Research Article

Rapid Purification and Procoagulant and Platelet Aggregating Activities of Rhombeobin: A Thrombin-Like/Gyroxin-Like Enzyme from Lachesis muta rhombeata Snake Venom

Figure 2

Enzymatic, kinetic properties and thrombin-like activity of Rhombeobin L. m. rhombeata venom. (a) The Michaelis-Menten curve. (b) The Lineweaver-Burk (double-reciprocal) plot. (c) Inhibition of the amidolytic activity by chelating agents (EDTA and EGTA), reducing agent (DTT), serineproteinase-specific inhibitors (PMSF), and soybean trypsin inhibitor (SBTI). (d) Minimum coagulant dose of Rhombeobin over citrated plasma (MCD-P) and fibrinogen bovine solution (2 mg/mL, MCD-F). The results of all experiments are the mean ± SEM of three determinations ( ).
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