BioMed Research International / 2013 / Article / Tab 2

Research Article

Responses of Growth Performance and Proinflammatory Cytokines Expression to Fish Oil Supplementation in Lactation Sows’ and/or Weaned Piglets’ Diets

Table 2

Oligonucleotide polymerase chain reaction primers.

Gene1Accession no.Primer sourcePrimer sequences ( )OrientationProduct size, bp (°C)

IL-1βM86725PigATTCGAGTCTGCCCTGTAForward14754
TCTGGGTATGGCTTTCCTReverse
IL-6 M80258PigGCATTCCCTCCTCTGGTCForward9358
ATAGTGTCCTAACGCTCATReverse
TNF-αAY572787PigCTCCCTCTTTGTCTCCTCCForward7754
GCATTGGCATACCCACTCTReverse
-actin SSU07786PigGGACTTCGAGCAGGAGATGGForward233
GCACCGTGTTGGCGTAGAGGReverse

IL-1β: interleukin 1 , IL-6: interleukin 6, TNF-α: tumor necrosis factor-α.
: optimal PCR annealing temperature.

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