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BioMed Research International
Volume 2013 (2013), Article ID 909613, 15 pages
Research Article

Inflammatory Bowel Disease Therapies and Gut Function in a Colitis Mouse Model

1School of Women’s and Children’s Health, University of New South Wales, Randwick, Sydney, NSW 2031, Australia
2School of Biotechnology and Biomolecular Sciences, University of New South Wales, Randwick, Sydney, NSW 2052, Australia
3Department of Gastroenterology, Sydney Children’s Hospital, Randwick, Sydney, NSW 2031, Australia
4Department of Pathology, Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North 4442, New Zealand
5Paediatric Gastroenterology, Christchurch Hospital, Christchurch 8140, New Zealand
6Department of Paediatrics, University of Otago, Christchurch, Christchurch 8140, New Zealand

Received 30 April 2013; Revised 1 July 2013; Accepted 1 July 2013

Academic Editor: David Bernardo

Copyright © 2013 Lily Nahidi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Composition of Osmolite (Abbott Nutrition; per 100 mL), as used for polymeric formula (PF) in the current study, is provided in Supplementary Table 1. As mentioned in the materials and methods, all animals, except for Exclusive Enteral Nutrition (EEN) group, were fed standard chow which its chemical composition is summarized in Supplementary Table 2. Cecum and colon tissues, obtained from each mouse at necroscopy, were examined and assigned a histological injury score corresponding to the severity of inflammation observed using a scheme modified from Madsen et al [9]. Histological scores ranged from 0 (minimal injury) to 15 (maximal injury) by scoring 4 criteria including epithelial hyperplasia (0-3), lamina propria mononuclear infiltration (0-4), Neutrophil infiltration (0-4) and mucosal ulceration (0-4) as outlined in Supplementary Table 3. Following histological examination, association of the average histological injury score with the presence and/or absence of Helicobacter trogontum in colon tissue, obtained from Interleukin (IL)-10-deficient mice at week 2 and 4, were assessed and the results are summarized in Supplementary Table 4. As shown in Supplementary Figure 1, the presence and/or absence of H. trogontum in the colon of noninfected, infected and infected but treated mice was evaluated at week 2 and week 4 using H. trogontum 16S rRNA specific polymerase chain reaction (PCR).

  1. Supplementary Material