Research Article

Actin, RhoA, and Rab11 Participation during Encystment in Entamoeba invadens

Figure 7

Disruption of the actin cytoskeleton with CD and Y27632 blocked the rearrangement of the actin cytoskeleton strongly affecting the cyst formation. (a) Percentage of inhibition of encystment by CD (1 μM) and Y27632 (1 μM) treatment during 96 h. ((b), (c)) Decrease in the intensity of F-actin fluorescence as a consequence of CD and Y27632 treatment. (d) Cysts ultrastructure at 96 h produced in the absence (control) or the presence of Cytochalasin D (CD) and ROCK-2 inhibitor (Y27632). Bar = 2 μm.
919345.fig.007a
(a)
919345.fig.007b
(b)
919345.fig.007c
(c)
919345.fig.007d
(d)