CpG and Interleukin-15 Synergize to Enhance IFN-γ Production by Activated CD8+ T Cells
Figure 1
The synergistic effects of IL-15 and CpG enhance CD8+ T cell IFN- production. (a) Naïve CD8+ T cells were cocultured with live Thy1.2-depleted splenocytes and anti-CD3 (0.1 g/mL) with increasing concentrations of recombinant human IL-15. After 72 hours culture supernatants were harvested and tested by ELISA for IFN-. (b) CD8+ T cells and Thy1.2-depleted splenocytes were cocultured with anti-CD3 for 72 hours in the presence of IL-15 (1 ng/mL), CpG (0.5 M), or IL-15+CpG. Cell culture supernatants were then harvested and IFN- levels were determined by ELISA. (c) Intracellular cytokine staining and flow cytometric analysis comparing CD8 expression (-axis) versus IFN- production (-axis) following 72 hour culture with IL-15+CpG. Numbers represent the percentage of CD8+ T cells that are IFN-+. Results are representative of three independent experiments and are expressed as . **; *; NS: not significant.
