2-Heptyl-Formononetin Increases Cholesterol and Induces Hepatic Steatosis in Mice
Figure 5
Adipocyte gene expression in C57BL/6 mice fed chow, cholesterol, or cholesterol supplemented formononetin or 2-heptyl-formonetin (C7F) for five weeks (Experiment 2). Gene expression measured by RT-PCR of Srebf1 (sterol regulatory element-binding protein-1c), Pparg (peroxisome proliferator-activated receptor γ), Cebpa (CCAAT/enhancer-binding protein α), Acaca (acyl-CoA carboxylase 1), Fasn (fatty acid synthase), Scd1 (stearoyl-CoA desaturase 1), Atgl (adipose triglyceride lipase), Ucp1 (uncoupling protein 1), and Emr1 (F4/80) in (A) eWAT, (B) iWAT, and (C) iBAT measured by RT-PCR. Data is normalised to 18S ribosomal RNA and presented relative to the expression in chow (). Graphs show mean ± SEM. Different letters (a, b) denote significant difference () between the groups.
