2-Heptyl-Formononetin Increases Cholesterol and Induces Hepatic Steatosis in Mice

Table 1

Body composition as shown by DXA scans of C57BL/6 mice before cholesterol feeding, after five weeks of initial cholesterol feeding and after three additional weeks where the mice were fed the cholesterol-enriched diet supplemented with either formononetin or 2-heptyl-formonetin (C7F) (Experiment  1). Data show mean ± SEM. Different letters (a, b, c) denote significant difference between the groups.

	Fat percentage	Body weight; (g)	Fat mass; (g)	Bone mineral concentration; (g)	Bone mineral density; (mg/cm^3)
Before test period
After acclimatisation (𝑛=30)	19.3 ± 7.8^a	16.7 ± 2.0^a	3.2 ± 1.8	0.3 ± 0.1^ab	640 ± 9^a
After initial cholesterol feeding (𝑛=23)	13.7 ± 6.8^b	24.3 ± 1.3^b	3.2 ± 1.7	0.3 ± 0.1^a	802 ± 10^b
After test period
Cholesterol (𝑛=25)	14.8 ± 8.2^b	28.0 ± 2.1^c	4.2 ± 2.6	0.4 ± 0.07^b	864 ± 6^c
Cholesterol + formononetin (𝑛=23)	13.1 ± 9.6^b	27.8 ± 2.3^abc	3.6 ± 2.8	0.4 ± 0.11^ab	839 ± 9^bc
Cholesterol + C7F (𝑛=24)	14.5 ± 5.5^b	26.1 ± 2.2^d	3.2 ± 1.7	0.4 ± 0.08^ab	825 ± 10^bc