Figure 1: Effects of water content and temperature on biodiesel yield by in situ transesterification of dried Chlorella pyrenoidosa biomass with distilled water. Reaction conditions: 1 g dried microalgae biomass (oil content 47%) with 4 mL methanol, 6 mL n-hexane and 0.5 M sulfuric acid for 120 min. Water content ranged from 0 to 90%. Temperature: 90, 120, and 150°C.