Direct Biodiesel Production from Wet Microalgae Biomass of Chlorella pyrenoidosa through In Situ Transesterification

Figure 3

Effects of methanol volume on the biodiesel yield by in situ transesterification of wet Chlorella pyrenoidosa biomass. Reaction conditions: 100 mg dry weight equivalent wet biomass (oil content 47%, water content 90%) with 6 mL n-hexane and 0.5 M sulfuric acid at 120°C for 120 min. Methanol volume: 1, 2, 4, 6, and 8 mL.