Developing a Clinical-Grade Cryopreservation Protocol for Human Testicular Tissue and Cells

Table 3

Recovery of viable cells positive for cell marker per gram of tissue.

Patient	SSEA4+			LHR+			VASA+
	Fresh cells	Frozen cells	Frozen tissue	Fresh cells	Frozen cells	Frozen tissue	Fresh cells	Frozen cells	Frozen tissue
1	382,500	133,392	126,144	459,000	222,571	523,697	10,128,600	2,894,214	5,642,505
2	642,000	271,081	540,099	1,883,200	378,955	5,003,870	8,089,200	5,820,763	24,075,066
3	215,200	141,845	215,335	1,102,900	67,546	1,069,848	10,733,100	1,939,856	11,733,700
4	829,602	259,954	108,069	3,402,562	1,201,910	644,282	28,455,915	11,992,882	6,595,656
5	1,085,311	426,618	599,564	1,141,806	1,865,490	3,814,563	25,813,056	15,991,324	6,356,519
Average	630,923	246,578	317,842	1,597,894	747,294	2,211,252	16,643,974	7,727,808	10,880,689
SEM	146,814	50,549	99,337	477,708	323,267	872,536	4,096,440	2,567,434	3,288,223
Percent recovery	N/A	39.1%	50.4%	N/A	46.8%	138.4%	N/A	46.4%	65.4%

The number of cells positive for each marker per gram of tissue isolated from fresh tissue cell isolation and recovered from either frozen cell or frozen tissue. Percent Recovery was calculated by dividing the average after cryopreservation by the average before cryopreservation. No significant differences were observed.