Depletion of Luminal Pyridine Nucleotides in the Endoplasmic Reticulum Activates Autophagy with the Involvement of mTOR Pathway
Figure 3
NADPH depletion induced by metyrapone enhances ER stress and influences the phosphorylation status of mTOR pathway proteins. HepG2 cells were treated with different amount of metyrapone (10 μM–100 μM) for 1 h at 37°C. (a) Cell lysates were analysed by Western blot by immunoreacting with antibodies against eIF2α-P, eIF2α, CHOP/Gadd153, GAPDH, and Gadd34, respectively. The relative density of Gadd34/GAPDH was plotted. (b) Cell lysates were analysed by Western blot by immunoreacting with antibodies against mTOR/FRAP-P, mTOR/FRAP, 4EBP1-P, 4EBP1, p70S6K-P, and p70S6K, respectively. The relative density of phosphorylated FRAP/GAPDH was represented.
