Hemizona Assay and Sperm Penetration Assay in the Prediction of IVF Outcome: A Systematic Review

Table 1

(a) Experimental parameters of studies investigating the validity of SPA. (b) Experimental parameters of studies investigating the validity of HZA.

(a)

Study group	Publication date	Study design	𝑛	Normal SP (𝑛)	Abnormal SP (𝑛)	Capacitation medium	Pre-incubation period
Margalioth et al. [23]	1983	Prospective	20	20	0	NR	NR
Wolf et al. [24]	1983	Prospective	24	24	0	Ham’s F-10 with 7,5% maternal serum	3 hrs
Foreman et al. [25]	1984	Prospective	22	14	8	Earle’s with 8% HSA	3 hrs
Ausmanas et al. [26]	1985	Prospective	54	42	12	BWW with 35 mg/mL HSA	5 hrs
Belkien et al. [27]	1985	Prospective	29	23	6	Ham’s F-10 with 10% maternal serum	6 hrs
Margalioth et al. [28]	1986	Prospective	134	124	10	mBWW with 35 mg/mL HSA	18 hrs
Corson et al. [29]	1987	Prospective	30	17	13	BWW with 5 mg/mL HSA	18–20 hrs
Kruger et al. [30]	1988	Retrospective	84	NR	NR	NR	NR
Coetzee et al. [31]	1989	Prospective	71	NR	NR	BWW	18–20 hrs
Ibrahim et al. [32]	1989	Prospective	59	35	24	BWW with 3.5 mg/mL HSA	3 hrs
Nahhas and Blumenfeld [33]	1989	Prospective	31	27	4	BWW with 36 mg/mL HSA	18 hrs
McClure et al. [34]	1990	Prospective	19	10	9	BWW with 6 mg/mL HSA	18 hrs
						0.4 mL of Human Follicular Fluid	0.5 hrs
Soffer et al. [35]	1992	Follow-up	241	NR	NR	TEST yolk buffer	18–22 hrs
Freeman et al. [36]	2001	Prospective	216	NR	NR	BWW with TEST yolk buffer	18–20 hrs

Abbreviations: 𝑛 (number of participants/patients), SP (Semen Parameters), BWW (Biggers, Whitten and Whittingham medium), HSA (Human Serum Albumin), NR (Not Reported).

(b)

Study group	Publication date	Study design	𝑛	Normal SP (𝑛)	Abnormal SP (𝑛)	Oocyte source	Oocyte preservation
Oehninger et al. [37]	1989	Prospective	28	17	11	SR ovarian tissue	Salt storage
Oehninger et al. [38]	1991	Prospective	37	15	22	IVF patient donation	Salt storage
Oehninger et al. [39]	1992	Prospective	44	31	13	SR ovarian tissue	Salt storage
Franken et al. [40]	1993	Prospective	112	58	54	SR ovarian tissue and IVF patient donation	Salt storage
Franken et al. [41]	1993	Prospective	48	28	20	SR ovarian tissue and IVF patient donation	Salt Storage
Gamzu et al. [42]	1994	Prospective	65	47	18	IVF patient donation	Salt Storage
Oehninger et al. [43]	1997	Prospective	196	138	58	SR ovarian tissue	Salt storage

Abbreviations: 𝑛 (number of participants/patients), SP (Semen Parameters), SR (Surgically Recovered).