Association between Interleukin-1 Gene Single Nucleotide Polymorphisms and Ischemic Stroke Classified by TOAST Criteria in the Han Population of Northern China

Table 2

The main characteristics of IL-1 gene and techniques used for screening.

	IL-1α	IL-1β
Polymorphism type	Single base C/T	Single base C/T
Polymorphism site	−889	−511
PCR primers
Upstream	5-GGGGGCTTCACTATGTTGCCCA; CACTGGACTAA-3	5-TGGCATTGATCTGGTTCATC-3
Downstream	5-GAAGGCATGGATTTTTACATAT; GAGCCTTCCATG-3	5-GTTTAGGAATCTTCCCACTT-3
PCR condition
Denaturation	94°C	94°C
Annealing	57°C	55°C
Extension	72°C	72°C
Cycles (𝑛)	35	35
Digestion	NCOI	AvaI
Fragment size (pb)	295	304