In Vivo Imaging of Leukocyte Recruitment to the Atheroprone Femoral Artery Reveals Anti-Inflammatory Effects of Rosuvastatin

Figure 1

Leukocyte recruitment in the femoral artery of ApoE−/− mice fed HF. (a) Snap-shot microphotographs of the intravital microscopy system (IVM) (left, ×200) and their quantification (right graph) (*𝑃<0.05, **𝑃<0.01). Arrow heads in microphotographs show adherent leukocytes in the femoral artery. (b) Total cholesterol level and triglyceride level in plasma of　ApoE−/− mice fed NC or HF for 2 weeks or 6 weeks (*𝑃<0.0001). (c) Relative mRNA expression levels of VCAM-1 and ICAM-1 in aortas of wt and ApoE−/− mice (*𝑃<0.05). (d) Leukocyte recruitment in following blockade of VCAM-1 by antibody (*𝑃<0.05). (e) Microphotograph of the aorta of ApoE−/− mice and wt stained by DHE (×200). (f) mRNA expression of gp91-phox and p22-phox in aorta of ApoE−/− mice (*𝑃<0.05).

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