BioMed Research International / 2013 / Article / Fig 7

Research Article

Revealing the Mechanism of In Vitro Wound Healing Properties of Citrus tamurana Extract

Figure 7

Transcription inhibition assay. Cdk-1 and -2 mRNA expression levels were measured following the treatment of fibroblasts cells with transcription inhibitor, DRB. Cells were pretreated with DRB for 2 h in serum free medium. Time 0 represents the time of HE addition. Total RNA was collected at various times thereafter and analysed by RT-PCR. Relative gene expressions were calculated plotted.

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