Antibacterial Activity of Defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in Endothelial Cells against Escherichia coli and Staphylococcus aureus
Table 1
Antibacterial effect of CM from BVE-E6E7 cells that express defensin PaDef from Persea americana var. .
Inhibition (%)b
Clones
E. coli
c
S. aureus
c
CM (total protein, g)
CM (total protein, g)
10
25
50
100
10
25
50
100
BVE3-PC
5.69
14.06
1.2
20.4
11.06
10.29
24.69
23.57
BVE3-C1
8.82
1.02
11.49
73.67*
4.75
4.33
38.87*
64.26*
BVE3-C2
10.37
9.74
10.51
69.64*
17.13
12.83
33.96*
63.73*
BVE3-C3
12.99
0.87
6.78
75.79*
11.64
14.35
30.86*
67.43*
BVE3-C4
23.56
15.44
14.91
65.37*
20.27
15.37
33.23*
54.59*
BVE3-C5
21.81
15.89
11.81
75.71*
13.77
6.14
30.25*
64.95*
BVE3-C6
20.26
16
7.73
78.08*
12.53
10.52
31.29*
63.86*
BVE3-C7
13.8
13.11
20.87
79.5*
11.97
3.87
31.07*
59.67*
BVE3-C8
22.15
13.4
9.56
70.88*
15.93
10.71
32.62*
58.41*
BVE3-C9
16.29
14.56
9.22
55.62*
1.47
14.45
27.02*
52.23*
Bacteria were incubated during 4 h at 37°C with CM.
bPercentage of inhibition considering the effect of CM from BVE-E6E7 cells as 100% for each strain.
cData represent the mean value ± SE of three independent experiments.
*Significant changes () compared to CM from BVE-E6E7 cells.
