Adhesion and Proliferation of Human Periodontal Ligament Cells on Poly(2-methoxyethyl acrylate)
Figure 7
Localization of nucleus, actin, and vinculin in adherent PDL cells on polymer surfaces. Scale bars: 10 μm. In the cross-section images, the top panel shows the nucleus (blue), the second panel shows the nucleus and actin (green), the third panel shows the nucleus and vinculin (red), and the bottom panel shows a merged image. The time points are 1 h, 4 h, 1 day, and 3 days. (a) PDL cells on PET. (b) PDL cells on PMEA. (c) PDL cells on PHEMA. White arrows indicate focal adhesions that were localized at the basal cell surface. Yellow arrows indicate nonfocal adhesions (non-FA) localized at the apical cell surface. White arrowheads indicate vinculin rods that were connected vertically and penetrated the adherent cell. White circles indicate vinculin fibers that were mainly oriented along the long axis of the adherent cells. (d) Schematic representation of vinculin fiber formation.
